TY  - BOOK
AU  - Walker, Jhon M.
TI  - Methods in molecular biology Vol. 1: : proteins
SN  - 978-0-89603-064-0
U1  - 574.88 - Biología molecular 
PY  - 1984///
CY  - Clifton: 
PB  - Humana Press, 
KW  - Biología Molecular
KW  - Proteínas
N1  - The lowry method for protein.--Determination of protein molecular weights by gel permeation high pressure.--Immunoaffinity purification of protein antigens.--Electrophoretic and chromatographic.--Peptide mapping by reverse--phase.--SDS polyacrylamide gel.--Gradient SDS polyacrylamide gel.--Acetic acid-urea polyacrylamide gel.--The in vivo isotopic labeling of proteins for polyacrylamide gel.--Two-dimensional.--Starch gel electrophoresis of proteins.--Isoeletric focusing in ultrathin.--High-sensistivity silver staining of proteins following polyacrylamide gel.-- Quantification of proteins on polyacrylamide gels.--Computer analysis of gel scans.--Drying gels.--Fluorography of polyacrylamide gels.--Recovery of proteins from dried polyacrylamide gel after fluorography.--The electrophoretic elution of proteins from polyacrylamide gel.- Transfer techniques in protein blotting.- Peptide mapping by thin-layer chromatography and high voltage.- In situ peptide mapping of proteins following polyacrylamide gel.- The dansyl method for identifying.--The dansyl-edman method for peptide sequencing.--Microsequencing of peptides and proteins with 4-N,N-dimethylaminoazobenzene-4.--Manual edman degradation of proteins and peptides.--Carboxyl-Terminal sequency determination of proteins and peptides with carboxypeptidase.--Immunization and fusion protocols for hybridoma production.--Immunofluorescence and immunoperoxidase screening of hybridomas.--Solid-phase screening of monoclonal antibodies.--The production of antisera.--Immunodiffusion in gels.--Crossed immunoelectrophoresis.--Radioiodination of proteins.--Radioimmunoassy.--Enzyme-Linked immunosorbant assay; 1984
ER  -